40 thousand litres of unleaded petrol spilled from the truck's two tanker trailers, some running into nearby waterways.
Firefighters used foam to supress flammable petrol fumes while the clean up took place.
At the height of the incident more than 60 firefighters and 10 trucks attended the scene 1km south of the Violet Town exit on the northbound section of the Hume Freeway.
Environmental crews are on scene collecting fuel and foam from surrounding creeks with the clean up expected to take days.
By 4pm on Friday 14 September, remaining fuel had been decanted from the damaged tanker while a second b-double truck was removed from the scene.
VicRoads was waiting for the wreckage to be removed before assessing the condition of northbound lanes to determine when they could reopen.
A crane was used to right the petrol tanker which had jacknifed and rolled onto it's side. A small car was also crushed in the accident which happened just after 11pm on Thursday 13 September.
The driver of the second truck and car escaped unhurt.
While atmospheric monitoring by CFA has found fumes were not at flammable levels, the community is warned the smell of petrol could remain in the air for some time.
For more information residents should go to www.cfa.vic.gov.au/warnings for the latest advice.
Operations Officer John Smith spoke to 3AW about the incident.Ops Officer John Smith on 3AW by cfavic
Read more about the incident at www.vicpolicenews.com.au